Name of module (course)

BIOCHEMISTRY

Code

ZBC

Field of study

dentistry

Profile

general academic

Level of studies

long-cycle studies

Specialisation

-

Mode of studies

full-time studies

Semester

III

Classes in the realm of  basic sciences

YES

Form of approval

exam

ECTS credit 6

Evaluation form

Form of study

Study hours

Total

Self-study

Contact hours

Teaching effects verification form

%

Lecture

30

10

20

Test

60

Seminar

50

30

20

Practical classes

80

30

50

Continuous observation, practical statement

30

Self-study

20

20

0

Preparation of materials and presentations

10

Total:

180

90

90

Total

100 %

Effects category

No.

Teaching effects for the course module

Teaching effect verification form

Field effect code

Area effect code

Notes

Knowledge

1.

knows the importance of major and trace elements in the processes occurring in the body, including the supply, absorption and transport

test

B.W.1

2.

knows the biochemical basis of the integrity of the human body

test

B.W.3

3.

knows the structure and functions of the major compounds present in the human body, in particular, the characteristics, functions, and energy metabolism reaction: proteins, nucleic acids, carbohydrates, lipids, enzymes and hormones;

test

B.W.4

4.

knows the role and significance of body fluids including saliva

test

B.W.6

5.

vital functions

test

B.W.19

6.

knows the principles of acid-base balance and the transport of oxygen and carbon dioxide in the body

test

B.W.21

7.

knows the principles of metabolism and nutrition

test

B.W.22

Skills

1.

refers to the phenomenon of chemical processes occurring in the oral cavity;

practical statement

B.U.1

2.

use of biological and ecological concepts in the context of a man - living environment

practical statement

B.U.4

Form of study

Course teachers

Lecture

Prof. dr hab. n. med. Sławomir Kasperczyk, dr hab. n. med. Jolanta Zalejska-Fiolka,

dr n. med. Urszula Błaszczyk, dr n. med. Ewa Romuk, dr hab. Michał Dobrakowski,

dr hab.n.med. Aleksandra Kasperczyk

Seminars

Prof. dr hab. n. med. Sławomir Kasperczyk, dr hab. n. med. Jolanta Zalejska-Fiolka,

dr n. med. Urszula Błaszczyk, dr n. med. Ewa Romuk, dr hab. Michał Dobrakowski,

dr hab.n.med. Aleksandra Kasperczyk

laboratory

Prof. dr hab. n. med. Sławomir Kasperczyk, dr hab. n. med. Jolanta Zalejska-Fiolka,

dr n. med. Urszula Błaszczyk, dr n. med. Ewa Romuk, dr hab. Michał Dobrakowski,

dr hab.n.med. Aleksandra Kasperczyk

Lecture

Semester III

Teaching methods

Lecture

No.

Topics

Hours

1.

Fundamentals of general and clinical enzymology.

2

2.

Electron Transport Chain and Krebs cycle.

2

3.

Carbohydrates metabolism part 1. The main pathways of carbohydrate, energetics.

2

4.

Carbohydrates metabolism part 2. Disorders of carbohydrate metabolism, diabetes mellitus

2

5.

Lipid metabolism part 1. Transport and storage of lipids. Biosynthesis and oxidation of saturated and unsaturated fatty acids; unsaturated fatty acid metabolism..

2

6.

Steroids biochemistry. Synthesis, transport and excretion of cholesterol. Bile acids.

2

7.

Metabolism of aminoacids, peptides and proteins

2

8.

Metabolism of nucleotides

2

9.

Structure of nucleic acids and expression of genetic information.

2

10.

Oxidative stress

2

Hours total:

20

Seminars

Semester III

Teaching methods

The teacher introduction, student presentations and discussion

No.

Topics

Hours

1.

Fudamentals

2

2.

Building, structure and function of peptides, proteins and enzymes.

2

3.

Tissue oxidation, phosphorylation, the Krebs cycle.

2

4.

Metabolism of carbohydrates.

2

5.

Lipid metabolism.

2

6.

Steroids biochemistry. Metabolism of cholesterol.

2

7.

Metabolism of proteins, proteolytic enzymes. Metabolism of selected amino acids.

2

8.

The metabolism of purine and pyrimidine nucleotides. Biosynthesis of the protein.

2

9.

Colloquim

4

Hours total:

20

Practical classes

Semester III

Teaching methods

The theoretical introduction and practical exercises on biological material, the interpretation of the results, reasoning

No.

Topics

Hours

1.

Safety in laboratory. Fundamentals in biochemistry.

5

2.

Characteristics and metabolism of plasma and serum proteins with particular reference to acute phase proteins. Methods for separation and analysis of proteins.

4

3.

Enzymology:

- Structure and properties of the enzymes.

- Methods for the enzymes classification with particular emphasis on the division of the enzyme.

- Kinetics of enzymatic reactions, the factors affecting the rate of enzymatic reactions including inhibition.

- Methods for the determination of enzyme activity (spectrophotometry, kinetic methods).

- Determination of enzymes: amylase, transferase, phosphatase.

- Introduction to the enzyme laboratory diagnostics.

16

4.

Carbohydrate metabolism:

- Characteristics of carbohydrates.

- Glucose homeostasis.

- Hyperglycemia and glycation of proteins.

- Hypoglycemia.

- Methods for the determination of glucose and glycated hemoglobin in the blood.

5

   5.

Protein-nitrogen and urea:

- The importance of the urea cycle and the diagnostic role of urea.

- Hyper and hypoazotemics.

- Methods for the quantitative determination of urea.

4

6.

Creatinine and uric acid:

- Biosynthesis and the role of creatine and phosphocreatine.

- Creatinine as diagnostics factor.

- Biosynthesis and the role of uric acid.

- Hyperuricemia.

- Methods for the determination of creatinine and uric acid in serum and urine.

4

7.

Saliva and Teeth:

- Production of saliva.

- Organic and inorganic components of saliva.

- Buffer capacity of saliva.

- The function of the digestive, secretory, and excretory salivary defense.

- Anatomical structure and chemical composition of teeth.

- Deposits of teeth engaging formations.

- Caries.

- Biochemical methods for determining pH and organic and inorganic components of saliva.

4

8.

Isolation of RNA from yeast

4

9.

RNA determination

Determination of ribose in RNA of yeast

Detection of purines

4

Hours total:

50

Self-study

Teaching methods

Expanding knowledge through search and familiarity with current information on the subject of the object. Strengthening the knowledge on the basis of an independent analysis of the available sources. (Source: Internet, scientific journals, books)

1.

Lippincott’s Illustrated Reviews Richard A. Harvey

2.

Harper Biochemistry – Murray R.K., Granner D.K., Mayes P.A., Rodwell V.W., PZWL Warsaw

1.

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko. PWN, Warsaw. Biochemistry.